normal human breast epithelial cell line mcf12a Search Results


mcf12a  (ATCC)
96
ATCC mcf12a
Mcf12a, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non tumorigenic breast epithelial cell line mcf 12 a  (ATCC)
94
ATCC non tumorigenic breast epithelial cell line mcf 12 a
Non Tumorigenic Breast Epithelial Cell Line Mcf 12 A, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell culture human cell lines  (ATCC)
99
ATCC cell culture human cell lines
Cell Culture Human Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mda mb 436 htb 130 normal human breast epithelial cell lines mcf 12a  (ATCC)
97
ATCC mda mb 436 htb 130 normal human breast epithelial cell lines mcf 12a
Mda Mb 436 Htb 130 Normal Human Breast Epithelial Cell Lines Mcf 12a, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3598 huvec atcc cat  (ATCC)
99
ATCC 3598 huvec atcc cat
3598 Huvec Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcf-12a healthy human mammary epithelial cells  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications mcf-12a healthy human mammary epithelial cells
Growth inhibition and cell cycle arrest by MitoQ or CoQ10. A and B, an SRB dye-based assay was used for measuring cell viability following increasing concentrations of either MitoQ (left) or CoQ10 (right) after 72 h. Breast cancer cell lines (MDA-MB-231 or <t>MCF-7)</t> or healthy breast epithelial cells <t>(MCF-12A)</t> were treated as indicated. B, a colony formation assay was performed on MDA-MB-231 cells incubated with MitoQ. Cells were incubated with MitoQ for 6 h, followed by replacement of medium and replating for a colony formation period of 7 days. C, MDA-MB-231 cells were treated with either MitoQ or CoQ10, as indicated, for 48 h. The cells were fixed and stained with PI followed by flow cytometric analysis. The percentage of cells in each cell cycle phase was quantitated and is provided in each panel. D, the phosphorylation of Chk1 and Chk2 kinases was assayed using confocal microscopy. Representative images of increased nuclear foci corresponding to phospho-Chk1-Ser317 (pChk1-S317), phospho-Chk2-Thr68 (pChk1-T68), and cytoplasmic phospho-Chk1-Ser345 (pChk1-S345) after 0.1 μm MitoQ or 0.1 μm doxorubicin (Dox) treatment (24 h) are shown. Error bars, S.D.
Mcf 12a Healthy Human Mammary Epithelial Cells, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human immortalized mammary epithelial cell lines (mcf-12  (iCell Gene Therapeutics)
90
iCell Gene Therapeutics human immortalized mammary epithelial cell lines (mcf-12
Growth inhibition and cell cycle arrest by MitoQ or CoQ10. A and B, an SRB dye-based assay was used for measuring cell viability following increasing concentrations of either MitoQ (left) or CoQ10 (right) after 72 h. Breast cancer cell lines (MDA-MB-231 or <t>MCF-7)</t> or healthy breast epithelial cells <t>(MCF-12A)</t> were treated as indicated. B, a colony formation assay was performed on MDA-MB-231 cells incubated with MitoQ. Cells were incubated with MitoQ for 6 h, followed by replacement of medium and replating for a colony formation period of 7 days. C, MDA-MB-231 cells were treated with either MitoQ or CoQ10, as indicated, for 48 h. The cells were fixed and stained with PI followed by flow cytometric analysis. The percentage of cells in each cell cycle phase was quantitated and is provided in each panel. D, the phosphorylation of Chk1 and Chk2 kinases was assayed using confocal microscopy. Representative images of increased nuclear foci corresponding to phospho-Chk1-Ser317 (pChk1-S317), phospho-Chk2-Thr68 (pChk1-T68), and cytoplasmic phospho-Chk1-Ser345 (pChk1-S345) after 0.1 μm MitoQ or 0.1 μm doxorubicin (Dox) treatment (24 h) are shown. Error bars, S.D.
Human Immortalized Mammary Epithelial Cell Lines (Mcf 12, supplied by iCell Gene Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
human immortalized mammary epithelial cell lines (mcf-12 - by Bioz Stars, 2026-07
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cell lines mcf12a atcc crl 10782 bt549 atcc htb  (ATCC)
99
ATCC cell lines mcf12a atcc crl 10782 bt549 atcc htb
Growth inhibition and cell cycle arrest by MitoQ or CoQ10. A and B, an SRB dye-based assay was used for measuring cell viability following increasing concentrations of either MitoQ (left) or CoQ10 (right) after 72 h. Breast cancer cell lines (MDA-MB-231 or <t>MCF-7)</t> or healthy breast epithelial cells <t>(MCF-12A)</t> were treated as indicated. B, a colony formation assay was performed on MDA-MB-231 cells incubated with MitoQ. Cells were incubated with MitoQ for 6 h, followed by replacement of medium and replating for a colony formation period of 7 days. C, MDA-MB-231 cells were treated with either MitoQ or CoQ10, as indicated, for 48 h. The cells were fixed and stained with PI followed by flow cytometric analysis. The percentage of cells in each cell cycle phase was quantitated and is provided in each panel. D, the phosphorylation of Chk1 and Chk2 kinases was assayed using confocal microscopy. Representative images of increased nuclear foci corresponding to phospho-Chk1-Ser317 (pChk1-S317), phospho-Chk2-Thr68 (pChk1-T68), and cytoplasmic phospho-Chk1-Ser345 (pChk1-S345) after 0.1 μm MitoQ or 0.1 μm doxorubicin (Dox) treatment (24 h) are shown. Error bars, S.D.
Cell Lines Mcf12a Atcc Crl 10782 Bt549 Atcc Htb, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human mammary gland derived cell lines  (ATCC)
99
ATCC human mammary gland derived cell lines
Growth inhibition and cell cycle arrest by MitoQ or CoQ10. A and B, an SRB dye-based assay was used for measuring cell viability following increasing concentrations of either MitoQ (left) or CoQ10 (right) after 72 h. Breast cancer cell lines (MDA-MB-231 or <t>MCF-7)</t> or healthy breast epithelial cells <t>(MCF-12A)</t> were treated as indicated. B, a colony formation assay was performed on MDA-MB-231 cells incubated with MitoQ. Cells were incubated with MitoQ for 6 h, followed by replacement of medium and replating for a colony formation period of 7 days. C, MDA-MB-231 cells were treated with either MitoQ or CoQ10, as indicated, for 48 h. The cells were fixed and stained with PI followed by flow cytometric analysis. The percentage of cells in each cell cycle phase was quantitated and is provided in each panel. D, the phosphorylation of Chk1 and Chk2 kinases was assayed using confocal microscopy. Representative images of increased nuclear foci corresponding to phospho-Chk1-Ser317 (pChk1-S317), phospho-Chk2-Thr68 (pChk1-T68), and cytoplasmic phospho-Chk1-Ser345 (pChk1-S345) after 0.1 μm MitoQ or 0.1 μm doxorubicin (Dox) treatment (24 h) are shown. Error bars, S.D.
Human Mammary Gland Derived Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+human+breast+epithelial+cell+line+mcf12a/pmc06110865-152-1-15?v=ATCC
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human mammary gland derived cell lines - by Bioz Stars, 2026-07
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human breast cancer cell lines  (ATCC)
97
ATCC human breast cancer cell lines
Growth inhibition and cell cycle arrest by MitoQ or CoQ10. A and B, an SRB dye-based assay was used for measuring cell viability following increasing concentrations of either MitoQ (left) or CoQ10 (right) after 72 h. Breast cancer cell lines (MDA-MB-231 or <t>MCF-7)</t> or healthy breast epithelial cells <t>(MCF-12A)</t> were treated as indicated. B, a colony formation assay was performed on MDA-MB-231 cells incubated with MitoQ. Cells were incubated with MitoQ for 6 h, followed by replacement of medium and replating for a colony formation period of 7 days. C, MDA-MB-231 cells were treated with either MitoQ or CoQ10, as indicated, for 48 h. The cells were fixed and stained with PI followed by flow cytometric analysis. The percentage of cells in each cell cycle phase was quantitated and is provided in each panel. D, the phosphorylation of Chk1 and Chk2 kinases was assayed using confocal microscopy. Representative images of increased nuclear foci corresponding to phospho-Chk1-Ser317 (pChk1-S317), phospho-Chk2-Thr68 (pChk1-T68), and cytoplasmic phospho-Chk1-Ser345 (pChk1-S345) after 0.1 μm MitoQ or 0.1 μm doxorubicin (Dox) treatment (24 h) are shown. Error bars, S.D.
Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+human+breast+epithelial+cell+line+mcf12a/pmc03525261-48-1-33?v=ATCC
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human breast cancer cell lines - by Bioz Stars, 2026-07
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study mcf 12a  (ATCC)
99
ATCC study mcf 12a
Growth inhibition and cell cycle arrest by MitoQ or CoQ10. A and B, an SRB dye-based assay was used for measuring cell viability following increasing concentrations of either MitoQ (left) or CoQ10 (right) after 72 h. Breast cancer cell lines (MDA-MB-231 or <t>MCF-7)</t> or healthy breast epithelial cells <t>(MCF-12A)</t> were treated as indicated. B, a colony formation assay was performed on MDA-MB-231 cells incubated with MitoQ. Cells were incubated with MitoQ for 6 h, followed by replacement of medium and replating for a colony formation period of 7 days. C, MDA-MB-231 cells were treated with either MitoQ or CoQ10, as indicated, for 48 h. The cells were fixed and stained with PI followed by flow cytometric analysis. The percentage of cells in each cell cycle phase was quantitated and is provided in each panel. D, the phosphorylation of Chk1 and Chk2 kinases was assayed using confocal microscopy. Representative images of increased nuclear foci corresponding to phospho-Chk1-Ser317 (pChk1-S317), phospho-Chk2-Thr68 (pChk1-T68), and cytoplasmic phospho-Chk1-Ser345 (pChk1-S345) after 0.1 μm MitoQ or 0.1 μm doxorubicin (Dox) treatment (24 h) are shown. Error bars, S.D.
Study Mcf 12a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+human+breast+epithelial+cell+line+mcf12a/pm39940603-77-7-12?v=ATCC
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megm  (Lonza)
90
Lonza megm
Growth inhibition and cell cycle arrest by MitoQ or CoQ10. A and B, an SRB dye-based assay was used for measuring cell viability following increasing concentrations of either MitoQ (left) or CoQ10 (right) after 72 h. Breast cancer cell lines (MDA-MB-231 or <t>MCF-7)</t> or healthy breast epithelial cells <t>(MCF-12A)</t> were treated as indicated. B, a colony formation assay was performed on MDA-MB-231 cells incubated with MitoQ. Cells were incubated with MitoQ for 6 h, followed by replacement of medium and replating for a colony formation period of 7 days. C, MDA-MB-231 cells were treated with either MitoQ or CoQ10, as indicated, for 48 h. The cells were fixed and stained with PI followed by flow cytometric analysis. The percentage of cells in each cell cycle phase was quantitated and is provided in each panel. D, the phosphorylation of Chk1 and Chk2 kinases was assayed using confocal microscopy. Representative images of increased nuclear foci corresponding to phospho-Chk1-Ser317 (pChk1-S317), phospho-Chk2-Thr68 (pChk1-T68), and cytoplasmic phospho-Chk1-Ser345 (pChk1-S345) after 0.1 μm MitoQ or 0.1 μm doxorubicin (Dox) treatment (24 h) are shown. Error bars, S.D.
Megm, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Growth inhibition and cell cycle arrest by MitoQ or CoQ10. A and B, an SRB dye-based assay was used for measuring cell viability following increasing concentrations of either MitoQ (left) or CoQ10 (right) after 72 h. Breast cancer cell lines (MDA-MB-231 or MCF-7) or healthy breast epithelial cells (MCF-12A) were treated as indicated. B, a colony formation assay was performed on MDA-MB-231 cells incubated with MitoQ. Cells were incubated with MitoQ for 6 h, followed by replacement of medium and replating for a colony formation period of 7 days. C, MDA-MB-231 cells were treated with either MitoQ or CoQ10, as indicated, for 48 h. The cells were fixed and stained with PI followed by flow cytometric analysis. The percentage of cells in each cell cycle phase was quantitated and is provided in each panel. D, the phosphorylation of Chk1 and Chk2 kinases was assayed using confocal microscopy. Representative images of increased nuclear foci corresponding to phospho-Chk1-Ser317 (pChk1-S317), phospho-Chk2-Thr68 (pChk1-T68), and cytoplasmic phospho-Chk1-Ser345 (pChk1-S345) after 0.1 μm MitoQ or 0.1 μm doxorubicin (Dox) treatment (24 h) are shown. Error bars, S.D.

Journal: The Journal of Biological Chemistry

Article Title: The Antioxidant Transcription Factor Nrf2 Negatively Regulates Autophagy and Growth Arrest Induced by the Anticancer Redox Agent Mitoquinone *

doi: 10.1074/jbc.M110.133579

Figure Lengend Snippet: Growth inhibition and cell cycle arrest by MitoQ or CoQ10. A and B, an SRB dye-based assay was used for measuring cell viability following increasing concentrations of either MitoQ (left) or CoQ10 (right) after 72 h. Breast cancer cell lines (MDA-MB-231 or MCF-7) or healthy breast epithelial cells (MCF-12A) were treated as indicated. B, a colony formation assay was performed on MDA-MB-231 cells incubated with MitoQ. Cells were incubated with MitoQ for 6 h, followed by replacement of medium and replating for a colony formation period of 7 days. C, MDA-MB-231 cells were treated with either MitoQ or CoQ10, as indicated, for 48 h. The cells were fixed and stained with PI followed by flow cytometric analysis. The percentage of cells in each cell cycle phase was quantitated and is provided in each panel. D, the phosphorylation of Chk1 and Chk2 kinases was assayed using confocal microscopy. Representative images of increased nuclear foci corresponding to phospho-Chk1-Ser317 (pChk1-S317), phospho-Chk2-Thr68 (pChk1-T68), and cytoplasmic phospho-Chk1-Ser345 (pChk1-S345) after 0.1 μm MitoQ or 0.1 μm doxorubicin (Dox) treatment (24 h) are shown. Error bars, S.D.

Article Snippet: MCF-12A healthy human mammary epithelial cells were obtained from BioWhittaker Clonetics (Walkersville, MD) and maintained in mammary epithelial cell growth medium ( 16 ).

Techniques: Inhibition, Colony Assay, Incubation, Staining, Confocal Microscopy